To assess whether all epithelial tumours of the gastrointestinal tract are derived from LGR5-positive stem cells, we crossed Lgr5-EGFP-IRES-creER(T2) mice, which express EGFP and Cre recombinase driven by the Lgr5 promoter, with CEA424-SV40-TAg mice, which develop pyloric neuroendocrine carcinomas of epithelial origin.

We then crossed the stem-cell-specific Lgr5-EGFP-IRES-creERT2 knockin mice to Apcflox/flox mice. In the resulting mice, Cre was. *These authors contributed 

Jun 21, 2012 using the knock-in allele Lgr5EGFP-Ires-CreERT2, we Distribution of stem cells marked by Lgr5-GFP and Paneth cells, marked by lysozyme  Lgr5陽性細胞でGFPとCreリコンビナーゼタンパク質を発現するLgr5-EGFP-Ires- CreERT2トランスジェニックマウスを、通称レインボーマウスと呼ばれる  Sep 20, 2012 We visualized Lgr5-EGFP+ve cells in neonate Lgr5-. EGFP-ires-CreERT2 kidney sections using anti-GFP immunohis- tochemistry (IHC) (Figure  To measure the overlap of Krt19+ cells with Lgr5+ cells, we performed Krt19 in situ hybridization on colonic (Figure 2A) tissues of Lgr5-EGFP-IRES-CreERT2  Methods Studies were performed in Lgr5−EGFP-IRES-creERT2 , Bmi1-CreERT2 , Hopx-CreERT2 , and TRE-H2BGFP::Hopx-CreERT2::lox-stop-lox-tdTomato  determined the effects of the chemoprotective FPA diet on miRNAs and mRNAs in colonic stem cells obtained from Lgr5-EGFP-IRES-creERT2 knock-in mice. determined the effects of the chemoprotective FPA diet on miRNAs and mRNAs in colonic stem cells obtained from Lgr5-EGFP-IRES-creERT2 knock-in mice. Använd 8 veckor gamla Actin-GFP mogna jomfru reportermöss, med vikter mellan Använd Procr Creert2-IRES-tdTomato / + (refererad till som Procr from neutral competition between symmetrically dividing Lgr5 stem cells. Lgr5-EGFP-IRES-creert2 (Lgr5-EGFP) möss tillhandahölls av Dr Eduard Batlle vid Institutet för forskning i biomedicin i Barcelona, ​​Spanien.

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15). All mice were crossed onto a C57BL/6 background for at least 10 generations. Although small and large intestines possess seemingly similar Wnt-driven leucine-rich repeat-containing G protein–coupled receptor 5 (Lgr5)+ adult epithelial stem cells, we report here that the two organs exhibit distinct mechanisms of tissue response to ionizing radiation. Employing Lgr5-lacZ transgenic mice and Lgr5 in situ hybridization, we found colonic epithelial stem cells (CESC Transcriptional differences between ISCs and their daughter cells can be explored by use of the Lgr5‐EGFP‐ires‐CreERT2 knock‐in (Lgr5‐ki) mouse (Supplementary Figure S1A; Barker et al, 2007). In this mouse model, GFP expression is driven from the Lgr5 locus, leading to highest GFP levels in Lgr5 + cells (GFP high). pressesLgr5-EGFPintheLgr5EGFP-Ires-CreERT2knock-inmice.The results showed that Lgr5-EGFP cells did not express the HBC markers, K14 and ICAM-1 (Fig.

H pylori colonize and manipulate the progenitor and stem cell compartments, which alters turnover kinetics and glandular hyperplasia. Bacterial ability to alter the stem cells has important implications for gastrointestinal stem cell biology and H pylori-induced gastric pathology.

Analysis of stem cell clones was performed at various time points after Cre-activation by tamoxifen in 10-week-old mice, after which the progeny of these Lgr5hi cells were mapped in 3D-reconstructed crypts. H pylori colonize and manipulate the progenitor and stem cell compartments, which alters turnover kinetics and glandular hyperplasia.

pressesLgr5-EGFPintheLgr5EGFP-Ires-CreERT2knock-inmice.The results showed that Lgr5-EGFP cells did not express the HBC markers, K14 and ICAM-1 (Fig. 1D,E). Instead, Lgr5-EGFP cells were localized immediately above the ICAM-1 or K14-positive HBCs, and displayed a distinct round morphology that was more characteristic of the GBC population

To induce the expression of the reporter gene, a single  Mus musculus, C57BL/6J, ET993, 3 to 9 (month), young, female, Lgr5-EGFP- IRES-creERT2, epithelium of small intestine, intestinal crypt stem cell, young  1 Aug 2012 To study the behavior of Lgr5+ cells within Apc-mutant adenomas, we then crossed Lgr5EGFP-Ires-CreERT2/Apcfl/fl mice with the R26R-Confetti  21 Jun 2012 Retracing of Lgr5+ adenoma cells. (A) Apc-deficient adenomas were allowed to develop for 24 days in Lgr5-EGFP-Ires-CreERT2/Apcfl/fl/R26R  Also Known As:Lgr5-EGFP-IRES-creERT2. Heterozygous mice harbor an Lgr5- EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function  were generated by crossing Lgr5–EGFP–ires–CreERT2 and. Klf5 flox/flox mice ( 4, 29). Because Cre–ERT2 fusion gene activity is inducible following tamoxifen  In 4 Lgr5-EGFP-IRES-CreERT2 Kras LSL-G12D mice studied between 2 and 8 months after tamoxifen. Figure 1. Hyperplastic and hyperproliferation changes in   Notch Signaling Regulates Gastric Antral LGR5 Stem Cell Function.

pressesLgr5-EGFPintheLgr5EGFP-Ires-CreERT2knock-inmice.The results showed that Lgr5-EGFP cells did not express the HBC markers, K14 and ICAM-1 (Fig. 1D,E). Instead, Lgr5-EGFP cells were localized immediately above the ICAM-1 or K14-positive HBCs, and displayed a distinct round morphology that was more characteristic of the GBC population Lgr5-eGFP-IRES-CreERT2 and Bmi1-CreER; Rosa26-YFP mice were treated with 12 Gy whole-body irradiation. By 2 d after irradiation, Lgr5-eGFP + ISCs and Olfm4 expression were completely lost from small intestine crypts ( Fig. 2 A , C , I – K , and L ), whereas there were no discernible quantitative effects on Bmi1-YFP + ISCs labeled with 1-d tamoxifen treatment ( Fig. 2 B and D ). Lgr5‐EGFP‐ires‐CreERT2 × Rosa26‐Tomato mice at postnatal day 40 and 41 (P40, P41) were divided into treated and untreated groups (n = 3).
Viral interactive limited

Lgr5 Gene Lgr5 Antibody. Hemsida.

1 A–F and N). To examine the relative contribution of Lgr5 vs. Bmi1 ISCs to tissue regeneration under steady-state conditions Lgr5-EGFP-Ires-CreERT2 and RosaSOR-LacZ mice (Jackson Laboratories, Bar Harbor, USA) were crossed to incorporate the LacZ reporter for lineage tracing upon administering tamoxifen .
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Although small and large intestines possess seemingly similar Wnt-driven leucine-rich repeat-containing G protein–coupled receptor 5 (Lgr5)+ adult epithelial stem cells, we report here that the two organs exhibit distinct mechanisms of tissue response to ionizing radiation. Employing Lgr5-lacZ transgenic mice and Lgr5 in situ hybridization, we found colonic epithelial stem cells (CESC

Lgr5 Gene Lgr5 Antibody.

quence of adenoma formation, we generated Lgr5-EGFP-. IRES-creERT2; APCflox/flox mice. Intraperitoneal injection of tamoxifen induced acute loss of APC in a 

Elise S. Demitrack1 Lgr5-EGFP-IRES-CreERT2; ROSA-LSL-Confetti; ROSANotchIC. Lgr5  quence of adenoma formation, we generated Lgr5-EGFP-. IRES-creERT2; APCflox/flox mice. Intraperitoneal injection of tamoxifen induced acute loss of APC in a  29 Mar 2012 immunostaining in Lgr5-EGFP-IRES-CreERT2 and wild-type mice. We compared Lrig1 and Lgr5 expression, as Lgr5 is an established SC  2 Feb 2018 The regenerative response of Lgr5-positive ISC was examined by lineage tracing assay using Lgr5-EGFP-ires-CreERT2-TdT mice with  Vav3−/− (Lgr5 APC V2V3) and Lgr5-EGFP-IRES-creERT2 Apcfl/fl.

Heterozygous mice harbor an Lgr5- EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function  Jun 12, 2019 (B-H) Embryonic liver organoids were generated from sorted Lgr5-GFP+ hepatoblasts isolated from E10.5-E12.5 Lgr5-EGFP-IRES-creERT2  quence of adenoma formation, we generated Lgr5-EGFP-. IRES-creERT2; APCflox/flox mice. Intraperitoneal injection of tamoxifen induced acute loss of APC in a  Lgr5-EGFP-IRES-. creERT2 mice were generated by homologous recombination in embryonic stem cells targeting an EGFP-IRES-creERT2 cassette to the ATG of   created miR-34aflox/flox mice/Lgr5-GFP-IRES-CreERT2 mice. cells, we cultured mouse intestinal cells from Lgr5-EGFP-IRES-CreERT2 mice in 3D.